ca2 indicator yc3 6 Search Results


ca2+ indicator yc3.6  (Nagai Nori USA INC)
90
Nagai Nori USA INC ca2+ indicator yc3.6
Ca2+ Indicator Yc3.6, supplied by Nagai Nori USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca2+indicator+yc3+6/yc2+60/10__1111_slash_j__1365___313x__2012__04917__x-59-4-22
Average 90 stars, based on 1 article reviews
ca2+ indicator yc3.6 - by Bioz Stars, 2026-09
90/100 stars
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ca 2 indicator yc3 6  (Addgene inc)
92
Addgene inc ca 2 indicator yc3 6
Reduced expression of GLP1R and GIPR in PDLSCs under multiple stress conditions. a Western blot assay show the expression of GLP1R and GIPR and PKA activity in PDLSCs respectively treated with cigarette, E-cig, hypoxia, LPS, alcohol, HG as well as MOF stimulation. Bubble plot indicates the quantitative analysis of the grayscale values of each band normalized to the β-actin (All western blot assays in this study were performed three duplications). Green bubbles indicate a significant decrease, whereas red bubbles indicate a significant increase compared to NC (gray bubble) ( P < 0.05) determined by Student’s t -test. Gray bubbles in other groups indicate no statistical significance compared with NC. b–d FRET-based biosensors detect the intracellular levels of cAMP ( b ), Ca 2+ ( c ), and PKA activity ( d ). CFP and YFP ratiometric images are shown as a pseudocolored green (CFP) and magenta (YFP) overlay (top). Quantifications of CFP/YFP emission ratio in the panels of sensor in different groups ( n = 20 in each group) (bottom). A ratio value more than 1 represents increased cAMP level, decreased Ca 2+ level, or inactivated PKA. e Western blot assay showing the expression of GLP1R and GIPR in PDLSCs derived from five healthy donors and five patients with periodontitis. The interpretation of bubble plot is illustrated in Fig. 1a. f , g Immunofluorescence show green fluorescence expression of the classical marker STRO-1, which is used to locate PDLSCs, and simultaneously demonstrate the co-localized red fluorescence expression of GLP1R ( f ) and GIPR ( g ) in NC (top) and PD (bottom) in vivo. H&E staining (right panel) is first performed to guide the identification of periodontal ligament tissue at second molar of NC and PD in serial sections, followed by immunofluorescence detection. Scale bar, 20 μm. Histogram show the differential positive expression rate of GLP1R or GIPR in STRO-1 positive cells compared between NC and PD. Green column indicate a significant decrease compared with NC (gray column) ( P < 0.05) determined by Student’s t -test (five fields for each group). NC, normal PDLSCs as negative control; Cig, cigarette; E-cig, E-cigarette; LPS, lipopolysaccharide; HG, high glucose; MOF, mild orthodontic force; FRET, Förster resonance energy transfer; HC, healthy control; PD, periodontitis
Ca 2 Indicator Yc3 6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca2+indicator+yc3+6/pCM5%2E58+(Plasmid+%2319375)/pmc12903609-104-10-20
Average 92 stars, based on 1 article reviews
ca 2 indicator yc3 6 - by Bioz Stars, 2026-09
92/100 stars
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yellow cameleon 3.6 (yc3.6)  (Nagai Nori USA INC)
90
Nagai Nori USA INC yellow cameleon 3.6 (yc3.6)
Reduced expression of GLP1R and GIPR in PDLSCs under multiple stress conditions. a Western blot assay show the expression of GLP1R and GIPR and PKA activity in PDLSCs respectively treated with cigarette, E-cig, hypoxia, LPS, alcohol, HG as well as MOF stimulation. Bubble plot indicates the quantitative analysis of the grayscale values of each band normalized to the β-actin (All western blot assays in this study were performed three duplications). Green bubbles indicate a significant decrease, whereas red bubbles indicate a significant increase compared to NC (gray bubble) ( P < 0.05) determined by Student’s t -test. Gray bubbles in other groups indicate no statistical significance compared with NC. b–d FRET-based biosensors detect the intracellular levels of cAMP ( b ), Ca 2+ ( c ), and PKA activity ( d ). CFP and YFP ratiometric images are shown as a pseudocolored green (CFP) and magenta (YFP) overlay (top). Quantifications of CFP/YFP emission ratio in the panels of sensor in different groups ( n = 20 in each group) (bottom). A ratio value more than 1 represents increased cAMP level, decreased Ca 2+ level, or inactivated PKA. e Western blot assay showing the expression of GLP1R and GIPR in PDLSCs derived from five healthy donors and five patients with periodontitis. The interpretation of bubble plot is illustrated in Fig. 1a. f , g Immunofluorescence show green fluorescence expression of the classical marker STRO-1, which is used to locate PDLSCs, and simultaneously demonstrate the co-localized red fluorescence expression of GLP1R ( f ) and GIPR ( g ) in NC (top) and PD (bottom) in vivo. H&E staining (right panel) is first performed to guide the identification of periodontal ligament tissue at second molar of NC and PD in serial sections, followed by immunofluorescence detection. Scale bar, 20 μm. Histogram show the differential positive expression rate of GLP1R or GIPR in STRO-1 positive cells compared between NC and PD. Green column indicate a significant decrease compared with NC (gray column) ( P < 0.05) determined by Student’s t -test (five fields for each group). NC, normal PDLSCs as negative control; Cig, cigarette; E-cig, E-cigarette; LPS, lipopolysaccharide; HG, high glucose; MOF, mild orthodontic force; FRET, Förster resonance energy transfer; HC, healthy control; PD, periodontitis
Yellow Cameleon 3.6 (Yc3.6), supplied by Nagai Nori USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca2+indicator+yc3+6/yellow+cameleon+3+6/pm23332153-74-17-28
Average 90 stars, based on 1 article reviews
yellow cameleon 3.6 (yc3.6) - by Bioz Stars, 2026-09
90/100 stars
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yellow cameleon yc3.6  (Baier labs)
90
Baier labs yellow cameleon yc3.6
Reduced expression of GLP1R and GIPR in PDLSCs under multiple stress conditions. a Western blot assay show the expression of GLP1R and GIPR and PKA activity in PDLSCs respectively treated with cigarette, E-cig, hypoxia, LPS, alcohol, HG as well as MOF stimulation. Bubble plot indicates the quantitative analysis of the grayscale values of each band normalized to the β-actin (All western blot assays in this study were performed three duplications). Green bubbles indicate a significant decrease, whereas red bubbles indicate a significant increase compared to NC (gray bubble) ( P < 0.05) determined by Student’s t -test. Gray bubbles in other groups indicate no statistical significance compared with NC. b–d FRET-based biosensors detect the intracellular levels of cAMP ( b ), Ca 2+ ( c ), and PKA activity ( d ). CFP and YFP ratiometric images are shown as a pseudocolored green (CFP) and magenta (YFP) overlay (top). Quantifications of CFP/YFP emission ratio in the panels of sensor in different groups ( n = 20 in each group) (bottom). A ratio value more than 1 represents increased cAMP level, decreased Ca 2+ level, or inactivated PKA. e Western blot assay showing the expression of GLP1R and GIPR in PDLSCs derived from five healthy donors and five patients with periodontitis. The interpretation of bubble plot is illustrated in Fig. 1a. f , g Immunofluorescence show green fluorescence expression of the classical marker STRO-1, which is used to locate PDLSCs, and simultaneously demonstrate the co-localized red fluorescence expression of GLP1R ( f ) and GIPR ( g ) in NC (top) and PD (bottom) in vivo. H&E staining (right panel) is first performed to guide the identification of periodontal ligament tissue at second molar of NC and PD in serial sections, followed by immunofluorescence detection. Scale bar, 20 μm. Histogram show the differential positive expression rate of GLP1R or GIPR in STRO-1 positive cells compared between NC and PD. Green column indicate a significant decrease compared with NC (gray column) ( P < 0.05) determined by Student’s t -test (five fields for each group). NC, normal PDLSCs as negative control; Cig, cigarette; E-cig, E-cigarette; LPS, lipopolysaccharide; HG, high glucose; MOF, mild orthodontic force; FRET, Förster resonance energy transfer; HC, healthy control; PD, periodontitis
Yellow Cameleon Yc3.6, supplied by Baier labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ca2+indicator+yc3+6/yellow+cameleon+yc3+6/pm37488718-91-21-38
Average 90 stars, based on 1 article reviews
yellow cameleon yc3.6 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Reduced expression of GLP1R and GIPR in PDLSCs under multiple stress conditions. a Western blot assay show the expression of GLP1R and GIPR and PKA activity in PDLSCs respectively treated with cigarette, E-cig, hypoxia, LPS, alcohol, HG as well as MOF stimulation. Bubble plot indicates the quantitative analysis of the grayscale values of each band normalized to the β-actin (All western blot assays in this study were performed three duplications). Green bubbles indicate a significant decrease, whereas red bubbles indicate a significant increase compared to NC (gray bubble) ( P < 0.05) determined by Student’s t -test. Gray bubbles in other groups indicate no statistical significance compared with NC. b–d FRET-based biosensors detect the intracellular levels of cAMP ( b ), Ca 2+ ( c ), and PKA activity ( d ). CFP and YFP ratiometric images are shown as a pseudocolored green (CFP) and magenta (YFP) overlay (top). Quantifications of CFP/YFP emission ratio in the panels of sensor in different groups ( n = 20 in each group) (bottom). A ratio value more than 1 represents increased cAMP level, decreased Ca 2+ level, or inactivated PKA. e Western blot assay showing the expression of GLP1R and GIPR in PDLSCs derived from five healthy donors and five patients with periodontitis. The interpretation of bubble plot is illustrated in Fig. 1a. f , g Immunofluorescence show green fluorescence expression of the classical marker STRO-1, which is used to locate PDLSCs, and simultaneously demonstrate the co-localized red fluorescence expression of GLP1R ( f ) and GIPR ( g ) in NC (top) and PD (bottom) in vivo. H&E staining (right panel) is first performed to guide the identification of periodontal ligament tissue at second molar of NC and PD in serial sections, followed by immunofluorescence detection. Scale bar, 20 μm. Histogram show the differential positive expression rate of GLP1R or GIPR in STRO-1 positive cells compared between NC and PD. Green column indicate a significant decrease compared with NC (gray column) ( P < 0.05) determined by Student’s t -test (five fields for each group). NC, normal PDLSCs as negative control; Cig, cigarette; E-cig, E-cigarette; LPS, lipopolysaccharide; HG, high glucose; MOF, mild orthodontic force; FRET, Förster resonance energy transfer; HC, healthy control; PD, periodontitis

Journal: Cellular & Molecular Biology Letters

Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs

doi: 10.1186/s11658-026-00867-2

Figure Lengend Snippet: Reduced expression of GLP1R and GIPR in PDLSCs under multiple stress conditions. a Western blot assay show the expression of GLP1R and GIPR and PKA activity in PDLSCs respectively treated with cigarette, E-cig, hypoxia, LPS, alcohol, HG as well as MOF stimulation. Bubble plot indicates the quantitative analysis of the grayscale values of each band normalized to the β-actin (All western blot assays in this study were performed three duplications). Green bubbles indicate a significant decrease, whereas red bubbles indicate a significant increase compared to NC (gray bubble) ( P < 0.05) determined by Student’s t -test. Gray bubbles in other groups indicate no statistical significance compared with NC. b–d FRET-based biosensors detect the intracellular levels of cAMP ( b ), Ca 2+ ( c ), and PKA activity ( d ). CFP and YFP ratiometric images are shown as a pseudocolored green (CFP) and magenta (YFP) overlay (top). Quantifications of CFP/YFP emission ratio in the panels of sensor in different groups ( n = 20 in each group) (bottom). A ratio value more than 1 represents increased cAMP level, decreased Ca 2+ level, or inactivated PKA. e Western blot assay showing the expression of GLP1R and GIPR in PDLSCs derived from five healthy donors and five patients with periodontitis. The interpretation of bubble plot is illustrated in Fig. 1a. f , g Immunofluorescence show green fluorescence expression of the classical marker STRO-1, which is used to locate PDLSCs, and simultaneously demonstrate the co-localized red fluorescence expression of GLP1R ( f ) and GIPR ( g ) in NC (top) and PD (bottom) in vivo. H&E staining (right panel) is first performed to guide the identification of periodontal ligament tissue at second molar of NC and PD in serial sections, followed by immunofluorescence detection. Scale bar, 20 μm. Histogram show the differential positive expression rate of GLP1R or GIPR in STRO-1 positive cells compared between NC and PD. Green column indicate a significant decrease compared with NC (gray column) ( P < 0.05) determined by Student’s t -test (five fields for each group). NC, normal PDLSCs as negative control; Cig, cigarette; E-cig, E-cigarette; LPS, lipopolysaccharide; HG, high glucose; MOF, mild orthodontic force; FRET, Förster resonance energy transfer; HC, healthy control; PD, periodontitis

Article Snippet: Intracellular Ca 2+ levels were measured using the genetically encoded Ca 2+ indicator YC3.6 (Yellow Cameleon 3.6, cat. no. 19375, Addgene).

Techniques: Expressing, Western Blot, Activity Assay, Derivative Assay, Immunofluorescence, Fluorescence, Marker, In Vivo, Staining, Negative Control, Förster Resonance Energy Transfer, Control